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dc.contributor.authorJerem, P
dc.contributor.authorHerborn, K
dc.contributor.authorMcCafferty, D
dc.contributor.authorMcKeegan, D
dc.contributor.authorNager, R
dc.date.accessioned2019-01-21T15:08:10Z
dc.date.available2019-01-21T15:08:10Z
dc.date.issued2015
dc.identifier.issn1940-087X
dc.identifier.issn1940-087X
dc.identifier.otherARTN e53184
dc.identifier.urihttp://hdl.handle.net/10026.1/13199
dc.description.abstract

Stress, a central concept in biology, describes a suite of emergency responses to challenges. Among other responses, stress leads to a change in blood flow that results in a net influx of blood to key organs and an increase in core temperature. This stress-induced hyperthermia is used to assess stress. However, measuring core temperature is invasive. As blood flow is redirected to the core, the periphery of the body can cool. This paper describes a protocol where peripheral body temperature is measured non-invasively in wild blue tits (Cyanistes caeruleus) using infrared thermography. In the field we created a set-up bringing the birds to an ideal position in front of the camera by using a baited box. The camera takes a short thermal video recording of the undisturbed bird before applying a mild stressor (closing the box and therefore capturing the bird), and the bird's response to being trapped is recorded. The bare skin of the eye-region is the warmest area in the image. This allows an automated extraction of the maximum eye-region temperature from each image frame, followed by further steps of manual data filtering removing the most common sources of errors (motion blur, blinking). This protocol provides a time series of eye-region temperature with a fine temporal resolution that allows us to study the dynamics of the stress response non-invasively. Further work needs to demonstrate the usefulness of the method to assess stress, for instance to investigate whether eye-region temperature response is proportional to the strength of the stressor. If this can be confirmed, it will provide a valuable alternative method of stress assessment in animals and will be useful to a wide range of researchers from ecologists, conservation biologists, physiologists to animal welfare researchers.

dc.format.extente53184-
dc.format.mediumElectronic
dc.languageen
dc.language.isoeng
dc.publisherMyJove Corporation
dc.subjectNeuroscience
dc.subjectIssue 105
dc.subjectAcute stress
dc.subjectblue tit
dc.subjectcapture
dc.subjectstress-induced hyperthermia
dc.subjectbody temperature
dc.subjectstress assessment
dc.titleThermal Imaging to Study Stress Non-invasively in Unrestrained Birds
dc.typejournal-article
dc.typeArticle
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/26575985
plymouth.issue105
plymouth.volume2015
plymouth.publisher-urlhttp://dx.doi.org/10.3791/53184
plymouth.publication-statusPublished online
plymouth.journalJournal of Visualized Experiments
dc.identifier.doi10.3791/53184
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA04 Psychology, Psychiatry and Neuroscience
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited States
dc.identifier.eissn1940-087X
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.3791/53184
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review


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